Figure 5.

WWOX regulates ER stress through IRE-1 and PERK. Western blots showing the expression of WWOX and ER stress regulators IRE-1 (ab48187 and ab37073 antibodies; abcam), PERK (no. 3192; Cell Signalling Technology), JNK (MAB2076 (Total) and AF1205 (phospho) antibodies; R&D Systems) (a) untreated or treated with 8 nM paclitaxel for 24 h in PEO1 cell lines in the presence or absence of WWOX. (b) SKOV-3 and OVCAR-4 cells, 72 h after treatment with siWWOX or no targeting control and 24 h after treatment with paclitaxel in 50 nM SKOV-3 and 20 nM OVCAR-4 cells. β-Actin has been used as a loading control. Cell viability was measured through SRB assay in (c) WWOX-8 and Vector-9 (d) SKOV-3 (72 h siRNA treatment) and (e) OVCAR-4 cells (72 h siRNA treatment) with IRE-1 inhibitor (KIRA6; 532281; Calbiochem) and PERK (GSK2656157; S7033; Selleckchem) alone or together in combination with paclitaxel. Cells were treated with 1 μg/μl inhibitors 2 h before paclitaxel treatment for 24 h. Triplicate experiments were performed