Figure 2.

AMD cybrids show decreased cellular viability and higher mitochondrial (mt) ROS production. (a) The MTT assay demonstrated reduced numbers of viable cells in AMD cybrid cultures (24 h, 18% decline, P=0.03, n=4); (48 h, 17% decline, P=0.03, n=4); (72 h, 18% decline, P<0.001, n=4). At 96 h, the cell numbers in both normal and AMD cybrids declined and were similar to each other (P=0.98, n=4) (Supplementary Table S4). (b) When measured with Trypan blue dye exclusion assay, cell viability at 72 h declined significantly (11%, P=0.002, n=5) in AMD cybrids compared to normal cybrids (Supplementary Table S4). (c) MitoSOX assay at 72 h demonstrated higher mitochondrial ROS production in AMD cybrids compared to normal cybrids (80%, P=0.003, n=5) (Supplementary Table S4). Data are represented as mean±S.E.M., normalized to normal cybrids (assigned value of 1). The endpoint for all experiments was 72 h