Figure 6.

HNG effects via intracellular (BAX) and extracellular pathways (gp 130 and phospho-JAK2) in AMD and normal cybrids as shown by western blot analyses: BAX protein levels were 145.9% higher (P=0.04, n=3–5) in untreated (UN)-AMD cybrids than untreated (UN)-normal (NL) cybrids (a). HNG-treated AMD cybrids had lower BAX protein levels (74.42%, P=0.002, n=3–5) compared to untreated-AMD cybrids (a). Untreated-AMD cybrids showed 44.4% lower gp130 protein levels compared to untreated-normal cybrids (P=0.03, n=3–4; b). HNG-treated AMD cybrids had 61.87% increased levels of gp130 protein compared to untreated-AMD cybrids (P=0.007, n=3–4; b) Phospho-JAK2 protein levels were 20.3% lower in untreated-AMD cybrids compared to untreated-normal cybrids (P=0.009, n=4; c). HNG-treated AMD cybrids showed 48.05% higher phospho-JAK2 protein levels than untreated-AMD cybrids (P=0.003, n=4; c) (Supplementary Table S9). Data are represented as mean±S.E.M., normalized to untreated-normal cybrids (assigned value of 1). The endpoint for all experiments was 72 h