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. 2017 Jul 20;8(7):e2951. doi: 10.1038/cddis.2017.348

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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HNG prevents loss of AMD mitochondria: (a) Confocal images of cybrids treated with mtGFP stain demonstrated increased mtGFP fluorescence in HNG-treated AMD cybrids. (b) In AMD cybrids, quantification graphs show that HNG increases mtGFP fluorescence by 194.3% (P<0.001, n=5) compared to AMD cybrids. The HNG-treated normal cybrids and untreated-normal cybrids showed similar levels of mtGFP staining (Supplementary Table S11). Scale bar=20 μm. Data are represented as mean±S.E.M., normalized to untreated-normal cybrids (assigned value of 1). The endpoint for all experiments was 72 h