Figure 2.
Inhibition of p70S6K activation induced by IGF-1 enhances cell death. FL5.12 BCL-XL/BAD S112A cells were deprived of IL-3 with or without rapamycin (20 ng/ml, Calbiochem) to initiate a death signal, whereas IGF-1 (50 ng/ml, Life Technologies) or IL-3 (10 ng/ml, Genzyme) was added back at 4 h before irreversible damage occurred. At each time point, viability was assessed by trypan blue exclusion. The IGF-1- or IL-3-dependent survival is compared with and without rapamycin. Data shown are representative of three independent experiments.