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. 2001 Aug 7;98(17):9666–9670. doi: 10.1073/pnas.171301998

Figure 4.

Figure 4

IGF-1-induced phosphorylation of BAD S136 is abolished in p70S6K-deficient cells. Serum was withdrawn from p70S6K+/+ or p70S6K−/− ES cells for 4 h (−IGF-1), followed by addition of IGF-1 (50 ng/ml) for 15 min (+IGF-1). Whole-cell extracts were immunoprecipitated with an anti-BAD antibody, followed by Western blot analyses with a phospho-S136-specific anti-BAD antibody or an antibody that recognizes BAD regardless of its phosphorylation state. Western blot analyses were also done by using an antibody that recognizes the phosphorylated form of AKT or an antibody that recognizes AKT regardless of its phosphorylation state. Data shown are representative of two independent experiments.