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. 2001 Aug 7;98(17):9666–9670. doi: 10.1073/pnas.171301998

Figure 5.

Figure 5

p70S6K suppresses BAD-mediated death in a S136-dependent manner. Rat-1a cells were transiently cotransfected with a luciferase reporter together with constructs expressing either BAD wild-type (Wt), S112A, S136A, or S112AS136A together with constructs expressing either p70S6K Wt or a kinase-dead (KD) mutant (Lys-100 → Gln) that does not bind ATP. Twenty-four hours after transfection, serum was withdrawn for 16 h. Luciferase activity (ordinate, arbitrary units) has been shown to represent the viability of the cells defined (9, 27), and this assay is representative of three independent experiments.