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CTCF depletion by siRNA enhances axonal extension capacity at P15. Depletion of CTCF by siRNA enhanced axonal extension in WT P15 DRG neurons, whereas there was no obvious effect on axonal extension in WT P3 DRG neurons (upper panels). Facilitated axonal extension in CTCF-depleted DRG neurons was reversed by cotransfection with eGFP-CTCF (lower panels). Representative images shown in left panels, means with standard errors in the right graph. *P<0.05 by ANOVA.
