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. 2017 Jun 12;6(7):1084–1095. doi: 10.1242/bio.026120

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — GATA6 depletion causes loss of definitive endoderm gene expression. (A) Bar graph showing results of gene ontology analysis using PANTHER of genes for which expression was affected by GATA6 depletion at day 3 of differentiation. (B) RT-qPCR analysis of GATA6 (+) and GATA6 (−) cells collected 5 days after induction. (C) Hierarchical cluster analysis of the mRNA levels of 464 genes contained within the definitive endoderm-enriched gene list in GATA6 (+) and GATA6 (−) cells at day 3, day 4 and day 5 of differentiation, as well as in GATA6–shRNA^indFlagGATA6 (GATA6 Rescue+Dox) cells at day 5 of differentiation. (D) RT-qPCR analysis of control and GATA6 shRNA rescue line with or without doxycycline. Error bars represent the standard error of the mean for the number of independent differentiations indicated. Statistical significance was determined via Student's t-test; *P<0.05, †P<0.01.