Fig. 1.

Luciferase assays and electrophoresis mobility-shift assay (EMSA). a Luciferase assays with vector constructs which were contained polymorphic sites, rs7294536 (−1502 A/G) and rs10877969 (− 649 A/G) in promoter region of the AVPR1A. The RLU values were denoted by mean and error bars by ± SD (n = 6). ***p < 0.001, **p < 0.01. b EMSA with oligonucleotides containing the rs7294536A or rs7294536G allele, c Western blotting with nuclear extract of T98G induced by 30 ng/ml of TNF-α. d Supershift assays with p65 and p50 antibodies and nuclear extract induced by 30 ng/ml TNF-α for 15 min