Fig. 4.

Transcript profiles of NR4A members in BeWo cells transfected with Nor-1, Nurr-1 or Nur-77 siRNA after forskolin treatment. BeWo cells were silenced for Nor-1, Nurr-1 or Nur-77 using the respective siRNA and transcript levels of all the three members were assessed using qRT-PCR after 0, 2 and 48 h of forskolin (25 μM) treatment. a, b and c – qRT-PCR analysis of the transcript levels of all three members in BeWo cells with Nor-1, Nurr-1 and Nur-77 knockdown, respectively. Each bar represents the relative expression after normalization with 18S rRNA, expressed as means ± s.e.m. of three independent experiments performed in triplicate. p ≤ 0.05 is considered statistically significant