Table 1.
Specific oligonucleotide primers used for PCR amplification
| SNP (gene) | PCR round | PCR primers | Product size (bp) | Digesting enzyme | Fragment size (bp) | |
|---|---|---|---|---|---|---|
| Digested (normal) | Digested (mutant) | |||||
| rs 334 (HBB) | First | 5′-AGAAAACATCAAGGGTCCCA-3′ (forward) | 926 | |||
| 5′-TCCATCTACATATCCCAAAGC-3′ (reverse) | ||||||
| Nested | 5′-TCCAAGGGTAGACCACCAGC-3′ (forward) | 445 | DdeI | 225, 220 | 445 | |
| 5′-GTGCCAGAAGAGCCAAGGAC-3′ (reverse) | ||||||
| rs 1050828 (G6PD) | Single | 5′-GTGGCTGTTCCGGGATGGCCTTCTG-3′ (forward) | 109 | NlaII | 109 | 63, 46 |
| 5′-CTTGAAGAAGGGCTCACTCTGTTTG-3′ (reverse) | ||||||
| rs 1800482 (NOS2) | Single | 5′-CATATGTATGGGAATACTGTATTTCAGGC-3′ | 573 | BsaI | 446, 127 | 573 |
| 5′-TCTGAACTAGTCACTTGAGG-3′ | ||||||