Table 1.

EC₅₀ values for the alanine scan, D-amino acid scan, and tail modification analogues of GBAP against the FsrC receptor^a

peptide name	EC50b [95% CI]c (nM)
GBAP	1.15 [0.825–1.59]
GBAP-Q1A	0.549 [0.299–1.01]
GBAP-N2A	0.704 [0.522–0.898]
GBAP-P4A	0.270 [0.202–0.360]
GBAP-N5A	341 [200–581]
GBAP-I6Af	> 1,000
GBAP-F7Af	> 1,000
GBAP-G8A	> 10,000
GBAP-Q9A	312 [222–437]
GBAP-W10A	246 [117–520]
GBAP-M11A	164 [93.8–288]
GBAP-q1d	0.211 [0.0798–0.599]
GBAP-n2	0.304 [0.168–0.552]
GBAP-s3	45.1 [17.0–120]
GBAP-p4	> 1,000
GBAP-n5	> 1,000
GBAP-i6	> 1,000
GBAP-f7	4.97 [3.12–7.77]
GBAP-q9	> 1,000
GBAP-w10	> 10,000
GBAP-m11	231 [129–414]
Ac-GBAP	1.50 [1.31–1.73]
GBAP-DesQ1	1.26 [1.15–1.39]
Ac-GBAP-DesQ1	1.44 [0.855–2.44]
GBAP-DesQ1N2 lactame,f	> 10,000
Ac-GBAP-DesQ1N2	1.01 [0.496–2.06]

^aSee Supporting Information for details of reporter strain and methods. See Supporting Information for plots of agonism dose response curves. All assays performed in triplicate. The full sequences for the peptides can be found in Table S-5.

^bEC₅₀ values determined by testing peptides over a range of concentrations.

^c95% confidence interval.

^dlower case letters indicate residues replaced with the D-enantiomer.

^eLactam (head-to-tail) cyclization.

^fWeak inhibitor.