Figure 3.

Effects of prim-O-glucosylcimifugin on lipopolyssacharide-induced NO and cytokine production. Raw 264.7 cells were incubated in a medium containing lipopolysaccharide (1 μg/mL) and various concentrations of prim-O-glucosylcimifugin (15, 50, and 100 μg/mL). Cells treated with dimethyl sulfoxide were set as control. The amount of nitrite (a), tumor necrosis factor-α (b), interleukin-6 (c), and interleukin-1β (d) in the medium was monitored at 24 h after exposure as described in Materials and Methods. All values are means ± standard deviation (n = 3). ^&&&P < 0.001 versus control; *P < 0.05, **P < 0.01, and ***P < 0.001 versus lipopolysaccharide-treated cells; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 versus lipopolysaccharide and 15 μg/mL prim-O-glucosylcimifugin-treated cells; ^$$P < 0.01 and ^$$$P < 0.001 versus lipopolysaccharide and 50 μg/mL prim-O-glucosylcimifugin-treated cells