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Effect of Tween-80 concentration, Liquid to material ratio, ultrasonic time and temperature on the responses. Extraction conditions were set as follows: (a) 25 mL/g, 25 min, (b) 25 mL/g, 25 min, 50°; (c) 7 % Tween-80, 25 min, 50°; (d) 7 % Tween-80, 25 mL/g, 50°; and (e) 7 % Tween-80, 25 mL/g, 25 min
