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. 1983;2(3):369–374. doi: 10.1002/j.1460-2075.1983.tb01432.x

Phosphorylation and actin activation of brain myosin.

B Barylko 1, A Sobieszek 1
PMCID: PMC555142  PMID: 11894951

Abstract

A method is described for obtaining brain myosin that shows significant actin activation, after phosphorylation with chicken gizzard myosin light chain kinase. Myosin with this activity could be obtained only via the initial purification of brain actomyosin. The latter complex, isolated by a method similar to that used for smooth muscle, contained actin, myosin, tropomyosin of the non-muscle type and another actin-binding protein of approximately 100,000 daltons. From the presence of a specific myosin light chain kinase and phosphatase in brain tissue it is suggested that the regulation of actin-myosin interaction operates via phosphorylation and dephosphorylation of myosin.

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Selected References

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