Figure 1. 3D reconstruction of high-resolution STED images of NKA isoforms.
3D reconstructions with volume rendering of mouse ventricular myocytes stained with NKA-α1 (Alexa Fluor 555) in blue and NKA-α2 (Oregon Green 488) in green from STED super-resolution imaging; scale bars are 10 μm. Both isoforms are present at the SSL and in transverse striations. Volumes were rendered with an adapted opacity transfer function to highlight SSL NKA isoform distribution. Fluorescence intensity scale (top) is in arbitrary units.