| H2O | Add to 50 μL |
| 5XPhusion HF buffer | 10 μL |
| 10mM dNTPs | 1 μL |
| IG-Forward 10μM | 1 μL |
| IG-Reverse 10μM | 1 μL |
| Template DNA | 5 μL |
| Polymerase | 0.5 μL |
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