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. Author manuscript; available in PMC: 2017 Aug 10.
Published in final edited form as: Nat Rev Microbiol. 2016 Feb 29;14(4):205–220. doi: 10.1038/nrmicro.2016.7

Figure 1. Example of electron cryotomography.

Figure 1

An intact Bdellovibrio bacteriovorus cell in standard media was plunge-frozen and imaged by electron cryotomography (ECT). The resulting tilt-series of images was reconstructed into a 3D tomogram. A slice through the reconstruction is shown (left panel), as well as a segmentation of visible cellular structures (right panel). To see the full reconstruction and segmentation, as well as fitting of crystal structures into electron microscopy densities, see Supplementary information S1 (movie).