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. Author manuscript; available in PMC: 2017 Aug 10.
Published in final edited form as: Science. 2017 May 26;356(6340):859–862. doi: 10.1126/science.aai9372

Fig. 4. TumiD regulates proteins critical for HEK293T-cell G1-to-S phase transition.

Fig. 4

(A) Flow cytometry analyses of the cell cycle demonstrating delayed G1-to-S phase progression in TSN KO#1 cells compared to WT cells. Cells were synchronized in G2/M using nocodazole, released from synchronization, and analyzed at the designated times. The percent (%) of cells in each cell-cycle phase is shown.

(B) RT-qPCR showing upregulation of TumiD targets in TSN KO#1 cells compared to WT cells. Cells were synchronized at G1/S using a double-thymidine block.

(C) RT-qPCR demonstrating that the levels of CDK2, CCND1, E2F1 and E2F2 mRNAs are downregulated in TSN KO#1 cells compared to WT cells when synchronized using a double-thymidine block, and that transfecting TSN KO#1 cells with miRNA inhibitors rescues the levels of CDK2, CCND1, E2F1 and E2F2 mRNAs.