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. 2017 Aug 8;7:164. doi: 10.3389/fonc.2017.00164

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Copyright © 2017 Wu, Aanei, Kesr, Picot, Guyotat and Campos Catafal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hematopoiesis-supporting potential of low-risk myelodysplastic syndromes (LR-MDS) and healthy controls (HC)-mesenchymal stromal cells (MSCs). BFU-E, CFU-G, and CFU-M isolated from healthy donors and LR-MDS were counted under a light microscope (A). Evaluation of mRNA expression of SDF-1 in LR-MDS MSC compared to HC (B). LR-MDS and HC-MSCs were cultured in adipogenic and osteogenic induction medium for 21 and 10 days, respectively, according to the protocol of the manufacturer. Then, the differentiation results were quantified by chemical staining (C). All experiments were performed in duplicate.