Table 2.
Frequency of endothelial progenitor cells, hematopoietic stem cells, and mesenchymal stem cells in the sternal bone marrow samples from patients with ischemic or valvular heart disease, in presence or absence of left ventricular dysfunction. Cells were analyzed by colony assay (CFU) and flow cytometry (FACS).
| Cell population (assay) | Heart disease | LV dysfunction | ||||
|---|---|---|---|---|---|---|
| IHD (n = 20) | VHD (n = 16) | p ∗ | Presence (n = 17) | Absence (n = 19) | p ∗ | |
| EPCs (CFU) | 1.9 (0.0–6.5) | 0.6 (0.0–7.5) | 0.675 | 1 (0–11) | 18 (4–44) | 0.038 |
| EPCs (FACS) | 174.1 ± 40.2 | 182.7 ± 51.7 | 0.611 | 171 ± 43.4 | 187 ± 45.6 | 0.341 |
| HSCs (CFU) | ||||||
| BFU-E | 6.5 (1–23) | 1 (0–19) | 0.411 | 21 (1–44) | 9 (0–48) | 0.727 |
| CFU-GM | 22 (6–46) | 15.5 (2–46) | 0.710 | 45 (9–95) | 44 (7–94) | 0.960 |
| CFU-GEMM | 54 (14–86) | 18 (1–91) | 0.313 | 67 (5–172) | 76 (7–266) | 0.881 |
| HSCs (CFU)—total colonies | 86 (22–154) | 48 (5–144) | 0.520 | 93 (14–309) | 167 (13–322) | 0.855 |
| HSCs (FACS) | 19.7 ± 6.6 | 17.6 ± 4.6 | 0.347 | 19.5 ± 6.3 | 17.9 ± 5.4 | 0.442 |
| MSCs (CFU) | 0.6 (0.3–0.9) | 0.2 (0–0.7) | 0.400 | 1 (1–1.5) | 3 (1–6) | 0.308 |
BFU-E: erythroid burst-forming units; CFU-GM: colony-forming units-granulocyte/macrophage: CFU-GEMM: multipotent myeloid stem cells; EPCs: endothelial progenitor cells; EV: ejection fraction; HSCs: hematopoietic stem cells; IHD: ischemic heart disease; LV: left ventricular; MSCs: mesenchymal stem cells; VHD: vascular heart disease. The results are presented as mean and standard deviation, or median and interquartile range. ∗p value, Student's t-test and chi-square or Mann–Whitney test.