Figure 3. . Neutrophil-derived nanovesicles target to inflamed vasculature induced by TNF-α.

(A) Western blot shows the presence of integrin β₂ on neutrophils (HL60 cells) and erythrocyte cell membrane nanovesicles (EV) as control for comparison of targeting ability of integrin β₂. (B) Intravital image of inflamed mouse cremaster venules shows the bound HL60 membrane-formed nanovesicles (HV) in green and the vasculature labeled with PECAM-1. (C) Image comparing binding of HV and EV to inflamed endothelium using intravital microscopy.

EV: Extracellular vesicle; HV: HL60 neutrophil-like vesicles; PECAM-1 (platelet endothelial cell adhesion molecule-1): endothelium marker

Reproduced with permission from [25].