. 2017 Jul 27;2017:3281670. doi: 10.1155/2017/3281670

Table 2.

Ameliorative effects of diosmin on tissue concentrations of lipid peroxidation and antioxidant markers in mice exposed to methotrexate.

Group	MDA (nmol/g)	NO (μmol/g)	GSH (mg/g)	GST (U/g)	GR (U/g)	GPx (U/g)	SOD (U/g)	CAT (U/g)
I. Hepatic tissue concentrations
Control	28.9 ± 1.8	53.3 ± 2.9	0.41 ± 0.02	49.13 ± 1.62	43.95 ± 1.45	37.78 ± 1.64	116.4 ± 3.99	41.39 ± 3.16
MTX	84.1 ± 3.07^∗	104.8 ± 2.9^∗	0.16 ± 0.02^∗	19.84 ± 1.74^∗	17.98 ± 0.93^∗	15.92 ± 1.75^∗	42.2 ± 1.28^∗	19.9 ± 2.24^∗
MTX-DM50	66.2 ± 4.5^∗^#	83.3 ± 2.5^∗^#	0.23 ± 0.02^∗	32.26 ± 2.08^∗^#	28.32 ± 1.99^∗^#	24.38 ± 1.94^∗^#	84.8 ± 3.59^∗^#	30.07 ± 2.52
MTX-DM100	35.2 ± 2.5^#	58.2 ± 3.6^#	0.33 ± 0.02^#	43.01 ± 1.28^#	37.45 ± 1.31^∗^#	28.70 ± 3.92^∗^#	106.04 ± 4.25^#	39.33 ± 2.37^#

II. Cardiac tissue concentrations
Control	49.47 ± 2.7	57.1 ± 2.2	26.8 ± 1.0	33.58 ± 1.78	5.76 ± 0.34	16.69 ± 0.96	57.32 ± 2.3	3.1 ± 0.14
MTX	138.7 ± 5.2^∗	158.7 ± 5.5^∗	12.35 ± 0.6^∗	16.65 ± 0.57^∗	2.45 ± 0.13^∗	7.20 ± 0.29^∗	29.58 ± 1.1^∗	0.73 ± 0.05^∗
MTX-DM50	89.6 ± 3.4^∗^#	97.5 ± 3.4^∗^#	16.44 ± 1.2^∗	24.42 ± 0.92^∗^#	3.67 ± 0.18^∗^#	10.62 ± 0.62^∗^#	44.29 ± 2.4^∗^#	1.3 ± 0.08^∗^#
MTX-DM100	56.3 ± 3.4^#	62.2 ± 3.4^#	24.3 ± 1.2^#	32.19 ± 1.13^#	5.59 ± 0.28^#	15.48 ± 0.18^#	53 ± 2.4^#	2.7 ± 0.2^#

III. Renal tissue concentrations
Control	36.8 ± 2.42	43.8 ± 1.47	9.04 ± 0.12	9.39 ± 0.34	13.06 ± 0.48	6.16 ± 0.14	57.2 ± 1.24	4.3 ± 0.27
MTX	77.3 ± 5.02^∗	87.6 ± 3.6^∗	4.52 ± 0.30^∗	5.23 ± 0.27^∗	6.51 ± 0.40^∗	2.96 ± 0.19^∗	27.55 ± 1.9^∗	2.05 ± 0.31^∗
MTX-DM50	49.5 ± 3.13^#	63.6 ± 2.45^∗^#	6.04 ± 0.43^∗^#	7.24 ± 0.20^∗^#	9.30 ± 0.28^∗^#	4.76 ± 0.16^∗^#	43.9 ± 3.9^∗^#	3.46 ± 0.27^#
MTX-DM100	39.2 ± 2.2^#	45.9 ± 1.04^#	8.25 ± 0.18^#	9.26 ± 0.26^#	12.08 ± 0.33^#	6.02 ± 0.15^#	53.5 ± 2.6^#	4.09 ± 0.28^#

Values are means ± SEM. ^∗Significant change at p < 0.01 with respect to the negative control group. ^#Significant change at p < 0.01 with respect to the MTX group as the positive control group. CAT: catalase; MDA: malondialdehyde; NO: nitric oxide; GPx: glutathione peroxidase; GR: glutathione reductase; GSH: glutathione; GST: glutathione S-transferase; SOD: superoxide dismutase.