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. 2017 Jul 27;2017:4386947. doi: 10.1155/2017/4386947

Figure 1.

Figure 1

Stabilized HIF-1α and HIF-2α in hypoxia (24 h, 1% O2) in parental wild-type (HIF-1α +/+) and HIF-1α −/− ES cells (a) and pharmacologically stabilized HIF-1α in wild-type ES cells (b) determined by western blot. Total level of β-actin was used as a loading control. Relative expressions of HIF-dependent ((VEGF (c), PGK1 (d)) and -independent (GDF15 (e)) genes in normoxia and hypoxia in wild-type and HIF-1α −/− ES cells. Data are presented as mean + SEM from at least three independent experiments. Statistical significance was determined by T test (∗P < 0.05).