Figure 6.

HPLC determination of specific 2-OH-E(+) (a) and nonspecific product E(+) (b) of HE oxidation in cells cultivated in normoxia or hypoxia or supplemented with GSH. Data are presented as mean + SEM from at least three independent experiments. Statistical significance was determined by one-way analysis of variance ANOVA and post hoc Bonferroni's multiple comparison test (^∗P < 0.05). Effect of GSH and β-ME supplementation on phosphorylation of MAPK/ERK, Akt, and STAT3 (c, d). Total level of β-actin was used as a loading control.