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. 2017 Jun 6;158(8):2427–2435. doi: 10.1210/en.2017-00349

Figure 1.

Figure 1.

ERα antagonist does not inhibit IGF1-induced uterine growth or gene regulation. (a) Ki67 proliferative marker immunohistochemistry of uterine samples from WT mice treated for 24 hours with saline V, E2, or IGF1 either alone (–ICI) or in combination with the ERα antagonist ICI 182,780 (+ICI). Scale bars = 60 µM. (b) RT-PCR shows that transcripts that are induced (Cdkn1a, Nr4a1) or repressed (Txnip, Sox4,) by E2 (2 hours) are also regulated by IGF1 (2 hours). ICI inhibits the E2-mediated upregulation, and partially inhibits the E2-mediated downregulation, but does not affect the IGF1-mediated regulation. Black bars: no ICI; gray bars: with ICI. Two-way analysis of variance with posttest *P < 0.05, ***P < 0.001, ****P < 0.0001 vs V; +P < 0.05, ++P < 0.01 no ICI vs with ICI; #### P < 0.0001 E2 vs IGF1. N = 4 or 5 for all samples.

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