Table 1.
Antibodies Used in This Study
| Peptide/Protein Target | Antigen Sequence | Name of Antibody | Manufacturer and Catalog No. | Species Raised in, Monoclonal or Polyclonal | Dilution Used | RRID |
|---|---|---|---|---|---|---|
| C3 | NA | Anti-C3 antibody | Abcam, Cambridge, UK, catalog no. ab97462 | Rabbit, polyclonal | 1:1000 (WB) or 1:100 (ICC) | AB_10679468 |
| Cleaved caspase-3 | NA | Cleaved caspase-3 (Asp175) | Cell Signaling Technology, Danvers, MA, catalog no. 9661 | Rabbit, polyclonal | 1:1000 | AB_2341188 |
| P-SAPK/JNK | NA | P-SAPK/JNK (Thr183/Tyr185) antibody | Cell Signaling Technology, Danvers, MA, catalog no. 9251 | Rabbit, polyclonal | 1:1000 | AB_331659 |
| JNK1 | NA | JNK1 (2C6) mouse mAb | Cell Signaling Technology, Danvers, MA, catalog no. 3708S | Mouse, monoclonal | 1:1000 | AB_1904132 |
| P-AKT | NA | P-Akt (Ser473) (D9E) XP® Rabbit mAb | Cell Signaling Technology, Danvers, MA, catalog no. 4060 | Rabbit, monoclonal | 1:10000 | AB_2315049 |
| AKT | NA | Akt antibody | Cell Signaling Technology, Danvers, MA, catalog no. 9272 | Rabbit, polyclonal | 1:5000 | AB_329827 |
| P-BAD | NA | P-Bad (Ser136) (D25H8) rabbit mAb | Cell Signaling Technology, Danvers, MA, catalog no. 4366 | Rabbit, monoclonal | 1:500 | AB_10547878 |
| BAD | NA | Bad antibody | Cell Signaling Technology, Danvers, MA, catalog no. 9292 | Unknown | 1:500 | AB_331419 |
| Glucagon | NA | Monoclonal anti-Glucagon antibody produced in mouse | Sigma, Bornem, Belgium, catalog no. G2654 | Mouse, monoclonal | 1:1000 | AB_259852 |
| Insulin | NA | Monoclonal anti-insulin antibody produced in mouse | Sigma-Aldrich, Bornem, Belgium, catalog no. I2018 | Mouse, monoclonal | 1:1000 | AB_260137 |
| Insulin (for histology) | NA | Polyclonal guinea pig anti-insulin antibody | DAKO, Glostrup, Denmark, catalog no. A056401-2 | Guinea pig, polyclonal | 1:250 | AB_2617169 |
| Insulin (for histology) | NA | Guinea pig anti-insulin polyclonal Antibody | Abcam, Cambridge, UK, catalog no. ab7842 | Guinea pig, polyclonal | 1:100 | AB_306130 |
| α-Tubulin | NA | Monoclonal anti–α-tubulin antibody | Sigma-Aldrich, Bornem, Belgium, catalog no. T9026 | Mouse, monoclonal | 1:5000 | AB_477593 |
| Anti-mouse IgG | NA | Peroxidase AffiniPure F(ab′)2 fragment donkey anti-mouse IgG (H+L) | Jackson ImmunoResearch Laboratories, West Grove, PA, catalog no. 715-036-150 | Polyclonal | 1:5000 | AB_2340773 |
| Anti-rabbit IgG | NA | Peroxidase AffiniPure F(ab′)2 fragment donkey anti-rabbit IgG (H+L) | Jackson ImmunoResearch Laboratories, West Grove, PA, catalog no. 711-036-152 | Polyclonal | 1:5000 | AB_2340590 |
| Goat anti-mouse IgG | NA | Goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 | Life Technologies, Carlsbad, CA, catalog no. A11029 | Goat, polyclonal | 1:1000 | AB_2534088 |
| Goat anti–guinea pig IgG | NA | Goat anti–guinea pig IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 | Life Technologies, Carlsbad, CA, catalog no. A11073 | Goat, polyclonal | 1:1000 | AB_2534117 |
| Donkey anti-rabbit IgG | NA | Alexa Fluor 488–AffiniPure donkey anti-rabbit IgG (H+L) antibody | Jackson ImmunoResearch Laboratories, West Grove, PA, catalog no. 711-545-152 | Donkey, polyclonal | 1:200 | AB_2313584 |
| Donkey anti–guinea pig | NA | DyLight 594–conjugated AffiniPure donkey anti–guinea pig | Jackson ImmunoResearch Laboratories, West Grove, PA, catalog no. 706-515-148 | Donkey, polyclonal | 1:200 | The product has been discontinued |
| Goat anti-rabbit IgG | NA | Goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 568 | Life Technologies, Carlsbad, CA, catalog no. A11036 | Goat, polyclonal | 1:1000 | AB_2534094 |
Abbreviations: ICC, immunocytochemistry; IgG, immunoglobulin G; mAb, monoclonal antibody; NA, not applicable; WB, western blot.