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. 2017 Jul 31;12(16):1911–1926. doi: 10.2217/nnm-2017-0140

Figure 4. . In vitro cytotoxicity and cell viability assays.

Figure 4. 

In vitro cytotoxicity assay (LDH) of (A) pentablock (PB) copolymers; (B) blank PB-NPs at different concentrations on D407, SV-40 and CCL20.2 cells. Less than 10% of LDH release was observed after 48 h and 12 weeks exposure, indicating negligible toxicity. In vitro cell viability assay (MTS) of: (C) PB copolymers; (D) blank PB-NPs at different concentrations on D407, SV-40 and CCL20.2 cells. The result demonstrated more than 90% cell viability after 48 hours exposure to PB copolymer.

LDH: Lactate dehydrogenase; MTS: (3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]-2Htetrazolium, inner salt; PB-NP: Pentablock-copolymer-nanoparticle.