Figure 5. . Dexamethasone-encapsulated-pentablock-copolymer-nanoparticle induced prolonged MYOC secretion from cultured human trabecular meshwork cells.

Representative western blot (WB) images show MYOC secretion over time in response to DEX (39.25 ng/ml), Con-NPs and DEX-PB-NPs treatment. Cells were exposed to NP preparations for entire 12-week period, while only exposed to DEX for first 4 weeks. Rows 1–12 show MYOC protein levels from week 1 to 12. Last row shows beta-actin from the same cells collected at end of 12-week period. n = 5. Four HTM cell strains (HTM120, 126, 134 and 136) showed similar MYOC secretion pattern as representative profile from HTM136 shown.

DEX-PB-NP: Dexamethasone-encapsulated-pentablock-copolymer-nanoparticle; HTM: Human trabecular meshwork; WB: Western blot.