Figure 6. . Quantification of MYOC secretion levels in response to dexamethasone-encapsulated-pentablock-copolymer-nanoparticle over time from human trabecular meshwork cells.

MYOC western blot images from all five HTM cell strains treated with DEX-NPs, Con NPs or DEX (39.25 ng/ml) for 12 time-points for each strain were digitized and quantified using ImageJ software whereby the band intensities were normalized to β-actin level observed for each individual cell strain. Cells were exposed to NP preparations for entire 12 weeks, while cells were only exposed to DEX for first 4 weeks. The relative MYOC secretion levels from DEX, Con-NPs and DEX-PB-NPs were compared with their individual controls at each time point. The combined data represent mean ± SE, n = 5. Symbols (* and ^#) indicate the significant differences compared with the Control group and control-NP group, respectively, using Mann–Whitney U Test.

DEX-PB-NP: Dexamethasone-encapsulated-pentablock-copolymer-nanoparticle; DEX: Dexamethasone; HTM: Human trabecular meshwork; NP: Nanoparticle.