Figure 8.

Effect of betaglycan on the binding and recruitment of TβRI. (A) SPR sensorgrams from a co-injection experiment in which a constant concentration of 1 μM TβRII and 1 μM BG_O-ZP was injected over immobilized TGF-β2, followed immediately by an injection of 1 μM TβRII and 1 μM BG_O-ZP with increasing concentrations of TβRI (serial 2-fold dilution from 2 to 0.063 μM TβRI). (B and C) SPR sensorgrams from a co-injection experiment performed in a manner identical to that described for panel A, but with no BG_O-ZP present in either first or second injection (B) or 1 μM BG_O used in place 1 μM BG_O-ZP during the first and second injection (C). Other details are as described for panel A. (D and E) Schematic depiction of how BG_O blocks the binding of TβRI and how it must be displaced to allow TβRI to bind, respectively.