Table 3. ITC Binding Data.
| sample cell component | TGF-β2 | TGF-β2TM/BGO-ZP | TGF-β2TM/TβRII |
| syringe component | BGO-ZP | TβRII | BGO |
| sample cell concentration (μM) | 5.40 | 16.7 | 10.0 |
| syringe concentration (μM) | 58.0 | 263 | 161 |
| buffer | 10 mM NaH2PO4 and 30 mM CHAPS (pH 7.4) | 10 mM NaH2PO4 (pH 7.4) | 25 mM glycine and 50 mM NaCl (pH 8.5) |
| N (sites) | 1.04 ± 0.04 | 1.04 ± 0.04 | 1.07 ± 0.02 |
| KD (nM) | 109 ± 56 | 510 ± 212 | 82 ± 26 |
| ΔH (kJ mol–1) | –52.6 ± 4.1 | –29.3 ± 1.8 | –38.8 ± 1.1 |
| ΔG (kJ mol–1) | –39.8 | –36.0 | –40.5 |
| –TΔS (kJ mol–1) | 12.8 | –6.7 | –1.7 |
| –8.6 |