Table 6. Binding Constants for Binding of TGF-β2 and TGF-β2TM to BGZP in the Presence and Absence of TβRII.
surface | analyte | KD (nM) | Rmax (RU) |
---|---|---|---|
TGF-β2 | BGZP | 450 ± 50 | 280 ± 10 |
TGF-β2 | BGZP (4 μM TβRII) | 600 ± 70 | 220 ± 10 |
TGF-β2TM | BGZP | 290 ± 40 | 310 ± 10 |
TGF-β2TM | BGZP (4 μM TβRII) | 5000 ± 1300 | 130 ± 20 |
TGF-β2 | BGZP-C | 450 ± 50 | 360 ± 10 |
TGF-β2 | BGZP-C (2 μM TβRII) | 600 ± 50 | 340 ± 10 |
TGF-β2TM | BGZP-C | 240 ± 30 | 620 ± 20 |
TGF-β2TM | BGZP-C (2 μM TβRII) | 2400 ± 200 | 320 ± 10 |