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Pyochelin biosynthesis. Pyochelin biosynthesis has been demonstrated biochemically using the three NRPS enzymes PchD, PchE, PchF, as well as the redutase PchG, along with building blocks DHB and cysteine, along with necessary substrates ATP, NADPH, and SAM. PchE contains an additional epimerization domain (E), while PchF contains an N-methyltransferase.
