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Effect of ACVTP on polymerase and exonuclease activities under processive conditions. UL30 was incubated with DNA15C (1 μM) in the presence of 0 μM or 10 μM dNTPs and varying concentrations of acyclovir triphosphate (0–80 μM). Aliquots of each reaction were analyzed at various times after initiating the reaction. (A) Phosphorimages of the products of DNA15C full extension and degradation using UL30. (B) Plot of exonuclease products as a function of ACVTP concentration at 6 min.
