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HSV pol does not efficiently polymerize dNTPs onto DNA containing a ribonucleotide at the 3′-terminus. UL30/UL42 (exo-) (50 nM) was incubated with DNAribo (1 μM) in the presence of 100 μM TTP and aliquots were taken out after 15 min. (A) Phosphorimages of the products. (B) Plot of products as a function of TTP concentration. Data were fit to the Michaelis–Menten equation and gave Vmax = 4.7 ± 0.4 (nM/min.) and KmdTTP = 163 ± 53 μM.
