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. 2017 Aug 7;216(8):2339–2354. doi: 10.1083/jcb.201512055

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© 2017 Cullati et al.

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Figure 1. — Nek depletion promotes cytokinesis failure. (A–C) HeLa (A), U2OS (B), and RPE1 (C) cells were treated with 10 nM of the indicated siRNAs for 48 and 72 h. Cells were fixed and stained for immunofluorescence, and the percentage of binucleated cells in >500 interphase cells was counted in three independent experiments. (D) Under conditions of Nek9 depletion, cytokinetic midbodies display disorganized PRC1 and tubulin staining that is consistent with depletion of citron kinase. Detergent-extracted HeLa cells stained with endogenous antibodies. n > 100 cytokinesis cells per condition in more than three independent experiments. *, P < 0.05 by Student’s t test; bar graphs show means ± SD. Bars: (main images) 20 µm; (insets) 5 µm.