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. 2017 Aug 7;216(8):2355–2371. doi: 10.1083/jcb.201610071

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© 2017 Ouenzar et al.

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Figure 3. — TLC1RNA partially colocalizes with DSB sites in rad52Δ cells. (A) FISH against TLC1 RNA in rad52Δ cells expressing Rfa1-GFP and treated with bleomycin (blm). Numbers in percentages represent the percentage of cells with the following phenotypes: no colocalization between a TLC1 RNA focus and Rfa1-GFP focus (top) or colocalization between a TLC1 RNA focus and Rfa1-GFP foci (bottom). Bar, 1 µm; n = 200 cells. (B) Dual FISH against TLC1 RNA and immunofluorescence on γ-H2A protein in rad52Δ cells after exposure or not to bleomycin (blm). Colocalization (indicated by arrowheads) was detected in 45 ± 7% of the cells. Bar, 1 µm; n = 200 cells.