Figure 4.

Genetic requirements for nucleoplasmic accumulation of TLC1 RNA after DNA damage in rad52Δ cells. (A) TLC1 RNA does not accumulate in the nucleoplasm in G2/M in the absence of DNA damage in rad52Δ, mre11Δ rad52Δ; xrs2Δ rad52Δ; tel1Δ rad52Δ; sml1Δ rad52Δ; sml1Δ mec1Δ rad52Δ and cdc13-2 rad52Δ cells. WT, rad52Δ, and sml1Δrad52Δ were used as control strains; n = 200–300 cells. (B) TLC1 RNA nucleoplasmic accumulation after induction of DNA damage requires Mre11 and Xrs2 of the MRX complex and Cdc13 and is regulated by Tel1. p-values are included in Table S2. Error bars represent ±SD; n = 150–300 cells. (C) FISH on TLC1 RNA in cdc13-2 rad52Δ cells treated or not with bleomycin. Arrowheads indicate the position of TLC1 RNA foci in cells. Bar, 1 µm. ***, P < 0.005 (two-tailed t test).