Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 7;216(8):2391–2407. doi: 10.1083/jcb.201611108

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Prusty et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Destabilization of Sm transcripts after extended inhibition of the late assembly phase. (A) Normalized fold change of transcripts encoding the indicated Sm proteins, SMN, and Gemin5, as well as U1 and U2 snRNAs in control cells (black bar) and SMN knockdown cells (gray bars), respectively, 144 h after doxycycline induction. (Top) Western blot analysis showing knockdown of SMN, with tubulin as loading control. (B) Normalized fold change of random transcripts (PI15, TOP3b, CPOX, hTR, and ASNS) that are unrelated to UsnRNP biogenesis as well as LSm encoding transcripts (LSm 2, 4,10, 11), PHAX and SNUP1. (C) qRT-PCR analysis of recovery of Sm encoding transcripts in control (black bars) and SMN knockdown (gray bars) cells upon treatment with 5-FU, an exosome inhibitor. Expression normalized to control cells treated with 5-FU. (Top) Western blot analysis showing knockdown of SMN, with tubulin as loading control. (D) Normalized fold change of transcripts encoding the indicated Sm proteins, SMN, and Gemin5, as well as U1 and U2 snRNAs in control cells (black bar) and SMN knockdown cells (gray bars) 168 h after doxycycline induction. (Top) Western blot analysis showing knockdown of SMN, with tubulin as loading control. Error bars indicate SE (n = 3, three independent biological experiments). **, P < 0.0005; *, P < 0.05, Student’s t test. GAPDH mRNA was used as a control for normalization.