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. 2017 Aug 7;216(8):2391–2407. doi: 10.1083/jcb.201611108

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© 2017 Prusty et al.

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Figure 8. — Sm proteins rescued from autophagy form insoluble aggregates. (A) Western blot analysis of lysates from HeLa cells treated with DMSO, compared with soluble extracts prepared from control and pICln siRNA–transfected cells, treated with autophagy inhibitor bafilomycin A, using antibodies specific to pICln and LC3 to monitor pICln knockdown and autophagy inhibition, with tubulin as loading control. (B) Western blot analysis of soluble (supernatant) and insoluble (pellet) fractions of control and pICln knockdown cells upon bafilomycin A treatment. Right top, amido black staining of histones after PVDF membrane transfer as loading control for the insoluble fraction; left top, tubulin (loading control for soluble fraction). (C) Quantification of the Western blots shown in B from n = 1 replicate.