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. 2017 Aug 7;216(8):2295–2304. doi: 10.1083/jcb.201612165

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© 2017 Hamdan et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 4. — The frequency of [PSI⁺] formation is increased in UPR and ERAD mutants. (A) The frequency of [PSI⁺] prion formation was quantified in wild-type, hac1, ire1, hrd1, and doa10 mutant strains. Data shown are the means of at least three independent biological repeat experiments ± SD. (B) Western blots probed with αSup35 or αPgk1. (C) The frequency of [PSI⁺] prion formation was quantified after the induction of the indicated chaperones (n = 3) ± SD. Significance is shown comparing strains grown on glucose media with strains grown on SGal media; *, P < 0.05; ***, P < 0.005 (n = 3).