Table 3.
Evaluation of the effects of CoCr29Mo6 ions on co-cultures of PBMCs and osteoblasts in comparison to single-cultured cells (PBMCs: n = 4; PBMCs; osteoblasts: n = 1 (same osteoblastic donor for all PBMC cultures)). PBMCs were transferred into standard culture plates allowing adherence over 72 h. Osteoblasts were seeded into cell culture inserts. After 24 h, cell culture inserts were transferred into the PBMC cultures and the respective cell culture medium was replaced by the ion solution. After 48 h and 96 h, RNA was isolated and reverse transcribed. Gene expression analyses of the differentiation markers and cytokines in the osteoblasts and PBMCs were done by qRT-PCR. Data are shown as median and minimum/maximum values (all in %). Significances between groups were calculated with the Mann-Whitney-U-Test (* p ≤ 0.05, treated vs. untreated).
| Differentiaton or Pro-Osteolytic Mediators | Osteoblasts | Adherent PBMCs |
|---|---|---|
| Col1A1 |
31 * (24; 49) |
- |
| RANKL |
2 (1; 5) |
- |
| OPG |
155 * (118; 206) |
- |
| RANK | - |
85 (47; 108) |
| TRAP5b | - |
10,649 * (9263; 12,099) |
| MMP1 |
128 (111; 307) |
285 * (212; 361) |
| TIMP1 |
2950 * (307; 8706) |
2573 * (978; 3490) |
| IL-6 |
5,306,604 * (3,684,037; 5,701,287) |
1093 * (700; 1315) |
| IL-8 |
4,089,451 * (1,769,434; 5,611,116) |
31,637 * (21,602; 46,144) |
| TNF-α |
489,124 * (194,021; 741,895) |
603 * (258; 887) |
| MCP-1 |
207,580 * (169,374; 267,626) |
654
(98; 1277) |