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Morphology of MC3T3-E1 cells cultured on PLLA surfaces. Cells were stained with Alexa Fluor 568 (red) and DAPI (4′,[6]-diamidino-2-phenylindole; blue). The scale bar represents 50 μm. Crystallization temperatures for normal spherulite samples (NSp) and ring-banded spherulite samples (BSp) were 150 and 143–147 °C, respectively. For preparing amorphous PLLA films (Amo), the molten state samples were quenched into ice water [60].
