Table 3.

Primer sequences and amplification conditions.

Gene	Primers Sequence	Conditions	Product Size (bp)
IL-8	5-ATGACTTCCAAGCTGGCCGTG-3′	5″ at 94 °C, 6″ at 55 °C,	297
	5-TGAATTCTCAGCCCTCTTCAAAAACTTCTC-3	12″ at 72 °C for 40 cycles
IL-1 α	5′-CATGTCAAATTTCACTGCTTCATCC-3′	5″ at 95 °C, 8″ at 55 °C,	421
	5′-GTCTCTGAATCAGAAATCCTTCTATC-3′	17″ at 72 °C for 45 cycles
TNF- α	5′-CAGAGGGAAGAGTTCCCCAG-3′	5″ at 95 °C, 6″ at 57 °C,	324
	5′-CCTTGGTCTGGTAGGAGACG-3′	13″ at 72 °C for 40 cycles
MMP-2	5′-TGACGGTAAGGACGGACTC-3′	5″ at 94 °C, 7″ at 57 °C,	342
	5′-TGGAAGCGGATTGGAAACT-3′	14″ at 72 °C for 40 cycles
MMP-9	5′-GCCAACTACGACACCGACGA-3′	5″ at 95 °C, 6″ at 56 °C,	310
	5′-CGCTGGTACAGGTCGAGTAC-3′	12″ at 72 °C for 40 cycles
HBD-2	5′-GGATCCATGGGTATAGGCGATCCTGTTA-3′	5″ at 94 °C, 6″ at 63 °C,	198
	5′-AAGCTTCTCTGATGAGGGAGCCCTTTCT-3′	10″ at 72 °C for 50 cycles