Figure 4.
Position-dependent effects of SR and hnRNP proteins in transcriptional and posttranscriptional control. (A) Schematic diagram of the constructs with and without carrying the MS2 binding sites in the second exon of the luciferase reporter. (B and D) Dual luciferase assays for the response to SR (panel B) and hnRNP (panel D) proteins tethered to the second exon of wild type (red) and mutant (blue) luciferase reporters. (C and E) RT-qPCR analysis of reporter RNA in cells transfected with individual SR (panel C) and hnRNP (panel E) proteins fused to GFP-MS2.