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. Author manuscript; available in PMC: 2017 Aug 10.
Published in final edited form as: Prostate. 2016 Feb 9;76(7):624–636. doi: 10.1002/pros.23155

Figure 4. TRAMP-C1 and TRAMP-C1scram-sh tumors induce differentiation of IMPs into M2 macrophages in the TME.

Figure 4

(A) The number of adoptively transferred IMPs, labeled with CFSE, in spleen and tumor was compared among the mice bearing TRAMP-C1, TRAMP-C1scram-sh and TRAMP-C1CRAMP-sh tumors. Data are shown as mean ± SE (n=3). *P < 0.01 ***P < 0.001 (B) The number of CFSE+ macrophages (Gr-1−, CD11b+, F4/80+) in the spleen and tumor was analyzed in mice bearing tumors with different level of cathelicidin-related antimicrobial peptide (CRAMP) expression. Results are presented as mean ± SE (n=3). *P < 0.01 (C) The phenotype of macrophages in the spleen and TME in mice bearing TRAMP-C1, TRAMP-C1scram-sh and TRAMP-C1CRAMP-sh tumors was determined. Data are shown as mean ± SE (n=3). (D) The number of macrophages differentiated from IMPs cultured with CM from TRAMP-C1 and TRAMP-C1CRAMP-sh cells, and M-CSF in vitro. *P < 0.05 (E) The number of M2 macrophages derived from IMPs cultured with conditioned medium from TRAMP-C1 and TRAMP-C1CRAMP-sh cells, and M-CSF in vitro. ***P < 0.001