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Polymerase chain reaction assay and pyrosequencing primers for detection of a genetic mutation in SLC2A9.
| Assay | Primer type | Primer sequence |
|---|---|---|
| Exon 5 SNP | Forward* | CTCACCCAAGGAGATCCG |
| Reverse | GTCAGTAGCCACGCTTACCTT | |
| Sequence | CCCGGTGAACACACCGA | |
| Promoter SNPs | Forward* | CCAGGCAACCCCTCCATTT |
| Reverse | ATGTGCTGAGTCCTTTTCACTGA | |
| Sequence | TCACCAATACGTAATTAGG |
Labeled with 5′-biotin.
