Figure 1.
Western blot analysis of SMAD4 and β-actin in colorectal cancer cell lines. Shown are the results of Western blot analysis using the B8 antibody against SMAD4, and a monoclonal anti-β-actin for four cell lines (LS180, C99, CX1, and COLO678). Note the complete absence of SMAD4 expression in CX1 and COLO678.